,

Neoscreen Galactose


SKU: MP34082
Labsystems Diagnostics NeoScreen Galactose kit is intended for the quantification of Galactose. present in blood samples dried on filter paper. The test is based on the combined determination of free and galactose liberated from the cleavage of the galactose1phosphate.

Galactosemia is an inherited metabolic abnormality comprising three distinctive clinical entities of the galactose pathway defects. Classic galactosemia is due to Dgalactose 1phosphateuridyltransferase (GALT) defect (1). The test principle is based on extraction of Galactose &amp Galactose1phosphate from dried blood spot followed by enzymatic conversion of Galactose1phosphate &amp Galactose. Two kinds of extraction methods (acidic and heat) can be used for extraction step. After extraction Galactose1phosphate converts to Galactose in a reaction catalyst by alkaline phophatase enzyme Then this Galactose &amp extracted Galactose from dried blood spot converts to Galactonolactone by Galactose Dehydrogenasis &amp NAD coenzyme. The resulted NADH reduces Tetrazolium salt indicator dye from yellow to pink or red color. The figure demonstrates the reaction cycles. Final color change is used for colorimetric measurement (490 nm ) spectrophotometrically.

SKU: MP34082 Categories: , Tags: ,

Based on 0 reviews

0.0 overall
0
0
0
0
0

Only logged in customers who have purchased this product may leave a review.

There are no reviews yet.